重组人 Tau dGAE (297-391) 模拟AD前体原纤维 (无肝素辅助聚集) Catalog No. SPR-502
产品详情：
| Product Name | Tau dGAE (297-391) 模拟AD前体原纤维 |
| Description |
重组人 Tau dGAE (297-391) 模拟AD前体原纤维 (无肝素辅助聚集) |
| Applications | WB, SDS PAGE, In vitro Assay |
| Concentration | 2 mg/ml 或 5 mg/ml |
| Conjugates | 无标签 |
| Nature | 重组的 |
| Species | 人 |
| Expression System | 大肠杆菌 (E. coli) |
| Amino Acid Sequence | IKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAE |
| Purity | >95% |
| Protein Length | 野生型tau全长蛋白 2N4R 的片段 (297 - 391aa) |
Properties：
| Storage Buffer | 10mM PB pH 7.4, 10mM DTT, 200mM MgCl2 |
| Storage Temperature | -80ºC |
| Shipping Temperature | 干冰. 运输: 该产品会与其他同订单产品分开发货. |
| Purification | 离子交换层析纯化 |
| Protein Size | 10.165 kDa |
| Cite This Product | Human Recombinant Tau dGAE (297-391) AD-mimic Pre-formed Fibrils (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-502) |
|
Certificate Of Analysis |
经SDS-PAGE & Nanodrop分析验证蛋白纯度 >95%. |
| Other Relevant Information | 为达到最佳效果请在使用前超声处理. 请参考官网上神经退行性疾病相关蛋白操作指南或产品说明书. 单体来源是目录号# SPR-316. |
Biological Description：
Alternative NamesTau aggregate, Tau PFFs, Tau PFF, Tau protein aggregate, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Truncated Tau Protein Aggregate, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, dGAE Tau Protein, Tau dGAEResearch AreasMT 相关蛋白, 神经元标记物, 神经纤维缠结&Tau, 神经退行性疾病, 细胞标记物, 轴突标记物, 微管, 神经生物学, 阿尔茨海默病, 细胞信号传导, 细胞骨架Swiss ProtP10636-8Scientific BackgroundFilamentous tau inclusions are a hallmark of many neurodegenerative diseases, including Alzheimer’s disease (AD) and Chronic Traumatic Encephalopathy (CTE), collectively called tauopathies. Advances in Cryo-EM have revealed that tau filaments isolated from individuals with a particular neurodegenerative disease share a distinct tau fold – i.e. an AD-isolated Tau filaments’ fold is distinct from a CTE-isolated Tau filaments’ fold (1-3). Utilizing Tau filaments with the correct disease-specific fold is an important goal towards better mimicking specific human diseases in cellular and in vivo models. Recent Cryo-EM studies have demonstrated that recombinantly generated Tau dGAE monomers will form the disease-isolated AD or CTE Tau filament folds under highly specific conditions in vitro (4, 5). StressMarq’s catalog# SPR-502 Tau dGAE (297-391) AD-mimic PFFs are purified and fibrilized under these exact published conditions that replicate the disease-isolated AD-fold (200 rpm at 37oC in 10 mM PB 10 mM DTT pH 7.4 200 mM MgCl2 for 48 hours).References1. Goedert, Eisenberg and Crowther. 2017. Propagation of Tau Aggregates and Neurodegeneration. Annu Rev Neurosci.
2. Fitzpatrick et al. 2017. Cryo-EM structures of tau filaments from Alzheimer’s disease. Nature.
3. Falcon et al. 2019. Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules. Nature.
4. Lovestam et al. 2022. Assembly of Recombinant Tau into Filaments Identical to those of Alzheimer’s disease and Chronic Traumatic Encephalopathy. eLife.
5. Lovestam et al. 2023. Disease-specific Tau Filaments Assemble via Polymorphic Intermediates. bioRxiv.
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